Biolegend, 566104, Biotinylated Recombinant Human LAIR2-Fc Chimera (carrier free)
25microg
Catalog Number: 566104
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| Source | Biotinylated human LAIR2-Fc Chimera, amino acids Gln22-Pro152 (Accession # Q6ISS4), with a C-terminal Fc and Avi tags, was expressed in 293E cells. Human LAIR2-Fc-Avi tag was site-specifically biotinylated by enzyme BirA. |
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| Molecular Mass | The 391 amino acid recombinant protein has a predicted molecular mass of approximately 43.0 kD. The DTT-reduced and non-reduced protein migrates at approximately 45-55 kD and 75-85 kD respectively in SDS-PAGE. The predicted C-terminal amino acid is Gln. |
| Purity | > 95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | 0.22 µm filtered protein solution is in PBS, pH 7.2 |
| Endotoxin Level | Less than 0.1 EU per µg of cytokine as determined by the LAL method. |
| Concentration | 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Storage & Handling |
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| Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at - 20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
| Activity | Biotinylated human LAIR2-Fc Chimera binds to immobilized collagen type I in a dose-dependent manner. EC50 of this binding is 0.175 – 1.4 ng/mL. |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | Ig-like protein |
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| Distribution | Expressed on PBMCs and CD4+ T cells |
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| Function | Blocks inhibitory signaling induced by LAIR1, tumor suppression and complement activation |
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| Ligand/Receptor | Collagens and collagen-like regions of C1q and of MBL (mannose binding lectin) |
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| Bioactivity | Biotinylated Human LAIR2-Fc Chimera binds to immobilized collagen type I in a dose-dependent manner. EC50 of this binding is 0.175 – 1.4 ng/mL. |
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| Cell Type | T cells |
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| Biology Area | Angiogenesis, Cell Adhesion, Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | Lebbink RJ, et al. 2008. Journal of Immunology. 180:1662. Lenting PJ, et al. 2010. PLoS One. 8:e12174. Olde Nordkamp MJ, et al. 2011. Arthritis and Rheumatism. 63:3749. Olde Nordkamp MJ, et al. 2014. J Innate Immun. 6:284. Wang P, et al. 2023. Clinical and Experimental Immunology. |
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| Gene ID | 3904 |
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| UniProt | View information about LAIR2 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | CD306, CD306 antigen, leukocyte-associated Ig-like receptor 2, eukocyte associated immunoglobulin like receptor 2, MGC71634 |
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