Biolegend, 571909, Recombinant Human IL-5 (ELISA Std.)
4pack
Catalog Number: 571909
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| Source | E. coli, approximately 26 kD |
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| Formulation | Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles. |
| Application | ELISA |
| Recommended Usage | Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 500 pg/ml to 4 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Application Notes |
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| This IL-5 protein is useful as a standard for a human IL-5 sandwich ELISA, using unlabeled JES1-39D10 antibody (catalog #500902) for capture and biotinylated JES1-5A10 antibody (catalog #501002) for detection. |
| Structure | Cytokine; dimer |
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| Bioactivity | Growth/differentiation of eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes |
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| Cell Sources | Mast cells, T cells, bone marrow stromal cells |
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| Cell Targets | Eosinophils |
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| Receptors | Heterodimer IL-5Rα (CD125); β-subunit (CDw131) in common with IL-3R, GM-CSFR |
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| Biology Area | Immunology, Cell Biology |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Takatsu K, et al. 1988. Immunol. Rev. 102:107. 3. Takatsu K. 1992. Curr. Opin. Immunol. 4:299. 4. Takatsu K. 1991. Microbiol. Immun. 35:593. |
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| Regulation | Upregulated by IL-2, platelet activating factor; downregulated by TGF-β |
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| Gene ID | 3567 |
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| UniProt | View information about IL-5 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | Interleukin-5, Eosinophil differentiation factor (EDF), Eosinophil colony stimulating factor (Eo-CSF), B cell growth factor-2 (BCGF-2), B cell differentiation factor for IgM (BCDF-m), IgA enhancing factor (IgA-EF), T cell replacing factor-1 (TRF-1) |
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