Biolegend, 585514, Cell-Vive™ GMP Recombinant Human EGF (carrier-free)
25microg
Catalog Number: 585514
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| Source | Human EGF, 54 amino acids Asn971-Arg1023 with an N-terminal Met (Accession# P01133) was expressed in E. coli. |
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| Molecular Mass | The 54 amino acid recombinant protein has a predicted molecular mass of approximately 6 kD. The DTT-reduced protein migrates at approximately 6 kD and non-reduced protein migrates at approximately 13 kD by SDS-PAGE. The N-terminal amino acid is Methionine. |
| N-terminalSequence Analysis | Met-Asn-Ser-Asp-Ser-Glu-(Cys)-Pro-Leu-Ser |
| Purity | ≥ 95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | 0.22 μm filtered protein solution is in PBS |
| Endotoxin Level | Less than or equal to 0.1 EU per μg protein as determined by the LAL method |
| Residual Host Cell Protein Content | ≤ 0.500 ng/μg by ELISA |
| Concentration | 25 μg and 100 μg sizes are bottled at 0.5 mg/mL |
| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at - 20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
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| Activity | Human EGF inhibits the proliferation of human epithelial A431 cells in a dose-dependent manner. The ED50 for this effect is 0.3 - 2.0 ng/mL. Human EGF induces the proliferation of NIH/3T3 cells in a dose-dependent manner. The ED50 for this effect is 0.025 – 0.125 ng/mL. The specific activity of Cell-Vive™ GMP Recombinant Human EGF (carrier-free) is ≥ 2.6 x 106 IU/mg when compared against the WHO International Standard for human EGF (NIBSC code: 91/530) in the A431 cell proliferation inhibition bioassay. |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Application References(PubMed link indicates BioLegend citation) | Henson ES and Gibson SB. 2006. Cell Signal. 18:2089. Burgess AW, et al. 2003. Mol. Cell. 12:541. Imai Y, et al. 1982. Cancer Res. 42:4394. Barnes DW. 1982. J. Cell. Biol. 93:1. Heo JS, et al. 2006. Am. J. Physiol. Cell. Physiol. 290:C123. |
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| Disclaimer | BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. |
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| Bioactivity | Human EGF inhibits the proliferation of human epithelial A431 cells in a dose-dependent manner. The ED50 for this effect is 0.3 - 2.0 ng/mL. Human EGF induces the proliferation of NIH/3T3 cells in a dose-dependent manner. The ED50 for this effect is 0.025 – 0.125 ng/mL. |
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| Cell Sources | Mammary gland cells, macrophages, gut epithelial cells, cells in the nervous system, and kidney |
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| Receptors | EGFR |
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| Antigen References | Arshad N, et al. 2018. J Biol Chem. 293:9555. PubMed Tam A, et al. 2019. Sci Rep. 9:3353. PubMed |
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| Regulation | EGF is a potent mitogen for many cells in culture, and in vivo, it induces the proliferation and differentiation of skin, cornea, lung, and trachea, among other tissues. Processing of pro EGF to mature EGF in different tissues is not equally efficient. The precursor is processed to mature EGF in the submaxillary gland, pancreas, small intestine, and mammary gland. In the submaxillary gland, EGF is fully processed, stored at secretory granules, and secreted in saliva. In kidney, EGF is present in unprocessed or intermediated forms on the cell surface. |
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| Gene ID | 1950 |
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| UniProt | View information about EGF on UniProt.org |
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| Other Names | Urogastrone (URG), HOMG4 |
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