Biolegend, 650053, Alexa Fluor® 594 Anti-Claudin 6 Antibody
25microg
Catalog Number: 650053
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Recombinant fragment of human Claudin-6 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | IHC-P - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Application Notes |
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| For use of this antibody in immunohistochemistry on formalin-fixed paraffin embedded tissues (IHC-P), we recommend antigen retrieval using Citrate Buffer, 10X (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704) |
| RRID | AB_3683487 (BioLegend Cat. No. 650053) AB_3683487 (BioLegend Cat. No. 650054) |
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| Structure | Consists of four transmembrane domains and two extracellular loops. It is a 220 amino acid length protein with a predicted molecular weight of 23 kDa. |
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| Distribution | Cellular membrane |
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| Function | Tight junctions and cell adhesion |
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| Antigen References | Reinhard K, et al. 2020. Science. 367(6476):446-453. Huan D, et al. 2021. Mol Med Rep. 24(3):677. Ushiku T, et al. 2012. Histophatology. 61(6):1043-56. Gowrikumar S, et al. 2019. Int J Mol Sci. 21:53. |
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| Gene ID | 9074 |
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| UniProt | View information about Claudin-6 on UniProt.org |
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| Clone | W22275B |
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| Regulatory Status | RUO |
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| Other Names | Claudin-6, Claudin 6, CLDN6, Skullin |
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| Isotype | Rat IgG2a, λ |
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