Cytiva, 17044003, Con A Sepharose 4B, 5 mL

Name: Con A Sepharose 4B, 5 mL
Brand: Cytiva
SKU: 17044003

Catalog Number: 17044003

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Category

Sample Filtration Devices

Pack Size5 mL

TagsChromatography resins Chromatography products Affinity resins - specific groups resin

CollectionsCytiva Chromatography Columns, Resins & Lab Glassware Cytiva Protein Analysis Equipment & Supplies Collection

Description

Con A Sepharose 4B with immobilized Concanavalin A is an affinity chromatography resin for capture of glycosylated biomolecules including glycoproteins and glycolipids. Stronger affinity for α-D-glucose than α-D-mannose
Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
Group-specific adsorbent for molecules containing sugars.
Highly suitable for isolation of cell surface glycoproteins from detergent-solubilized membranes
Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
Highly suitable for isolation of cell surface glycoproteins from detergent-solubilized membranes
Con A coupled to Sepharose 4B via cyanogen bromide activation
Con A Sepharose is Concanavalin A coupled to Sepharose 4B by the cyanogen bromide method. Concanavalin A (Con A) is a tetrameric metalloprotein isolated from Canavalia ensiformis (jack bean). Con A binds molcules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose is routinely used for separation and purification of glycoproteins, polysaccharides and glycolipids

Specifications

General

Avoid Using	Chelating agents such as EDTA, 8 M urea or solutions having a pH below 3 should be avoided as these conditions results in removal of manganese from the lectin with loss of activity as a result.
Chemical stability	Stable to commonly used aqueous buffers
Flow velocity	< 75 cm/h at 25 ˚C, HR 16/10 column, 5 cm bed height
Ligand	Concanavalin A (Con A)
Ligand Coupling Method	Cyanogen bromide activation
Ligand density	13 mg Con A/ml drained medium
Matrix	4% Agarose
Particle Size	45 µm-165 µm
Particle size, d 50V	~90 μm
Pressure/Flow Specification	Base matrix 70-140 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, 5 cm i.d.
Storage	2 to 8°C, 0.1 M acetate buffer pH 6, 1 M NaCl, 1 mM CaCl₂, 1 mM MnCl₂ and 1 mM MgCl, 20% Ethanol
pH stability, CIP	4–9
pH stability, operational	4–9

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