Answer:A narrow isoelectric focusing range of pH 2.5–5.
Answer:A 25 mL package of carrier ampholyte solution.
Answer:Select another pH interval when the proteins of interest have isoelectric points outside pH 2.5–5.
Cytiva Pharmalyte carrier ampholytes form a narrow pH 2.5–5 gradient in isoelectric focusing (IEF) applications, where proteins separate by their isoelectric points. The product is supplied as a 25 mL solution containing amphoteric polymeric components that distribute across the electric field and stabilize a reproducible pH gradient. The narrow acidic interval is suited to resolving proteins or charge variants whose expected isoelectric points fall within that range; it should not be selected for broadly basic proteins. Prepare the focusing matrix and sample using a validated IEF method, control ampholyte concentration and maintain temperature control because electrical heating can distort gradients. When using a gel or other focusing system, establish the recommended prefocusing and run conditions before loading analytical samples. Evaluate staining and downstream compatibility if proteins will be recovered or characterized after separation. This reagent is not a calibration buffer or a ready-made fixed-pH gel.
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