Cytiva, 17095601, Gelatin Sepharose 4B affinity resin, 25 mL
Catalog Number: 17095601
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Category
Pack Size25 mL
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| Chromatography technique | Group specific affinity |
|---|---|
| Certificate of Analysis | Yes |
| Chemical stability | Stable to commonly used aqueous buffers |
| Flow velocity | < 75 cm/h at 25 °C, HR 16/10 column, 5 cm bed height |
| Ligand | Bovine gelatin |
| Ligand Concentration | 4.5-8 mg gelatin/ml drained resin |
| Ligand Coupling Method | Cyanogen bromide activation |
| Ligand density | 4.5-8 mg gelatin/ml drained resin |
| Matrix | agarose, 6%, spherical |
| Particle size, d 50V | ~90 µm |
| Storage | 2 to 8°C, 20% Ethanol |
| pH stability, CIP | 3–10 |
| pH stability, operational | 3–10 |
Answer:Fibronectin, through affinity binding to immobilized gelatin.
Answer:25 mL of swollen affinity chromatography medium.
Answer:Gelatin-coupled Sepharose 4B based on 4% agarose.
Cytiva Gelatin Sepharose 4B is an affinity chromatography resin for isolating or removing fibronectin from biological samples such as plasma. Gelatin ligands are immobilized on a Sepharose 4B matrix composed of 4% agarose, providing selective interaction with fibronectin under suitable binding conditions. The swollen resin is supplied as 25 mL of chromatography medium for packing into a compatible column. The matrix has an average bead diameter of approximately 90 µm and a working pH range of 3–10. This is loose affinity resin rather than a prepacked column or free gelatin reagent.
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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