Cytiva, 17099804, Heparin Sepharose 6 Fast Flow resin, 5 L

Name: Heparin Sepharose 6 Fast Flow resin, 5 L
Brand: Cytiva
SKU: 17099804

Catalog Number: 17099804

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Category

Affinity Tag Purification Resins

Pack Size5 L

TagsChromatography resins Chromatography products Affinity resins - specific groups resin

CollectionsCytiva Chromatography Columns, Resins & Lab Glassware Cytiva Protein Analysis Equipment & Supplies Collection

Description

Heparin Sepharose 6 Fast Flow is an established BioProcess affinity resin for capture or intermediate purification of proteins with affinity for heparin.
Proven for industrial plasma protein purification of various proteins, including antithrombin III antibodies, and coagulation factors.
Based on Sepharose Fast Flow resins, which are well established in industrial downstream processing. Hydrophilic base matrix ensures low levels of nonspecific binding and low levels of host cell-derived impurities in the elution pool.
Heparin ligand provides enhanced coupling chemistry and chemically stable ligand attachment.
Fulfills industrial demands for security of supply, robust performance, and regulatory support.
Applications of heparin affinity chromatography
Cytiva's heparin resin allows for fast and reliable separations of biomolecules with an affinity for heparin, including antithrombin III antibodies, coagulation factors, and other plasma proteins. DNA-binding proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors can also be purified.
Chemically stable heparin purification resin
Heparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads, modified with sodium heparin. Immobilization is performed using chemically stable and oriented coupling, which delivers high binding capacity. The chemical stability of the heparin resin is solely dictated by the ligand.
Streamlined Heparin Sepharose Fast Flow scale-up
Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools.
Successful use in published research
This heparin resin is used in a variety of research applications, including:
Study of the biological activity of recombinant human lactoferrin in glycoengineered Pichia pastoris
Evaluation of the function of the MPH1 gene of saccharomyces cerevisiae in Okazaki fragment processing
Compatible empty columns – research scale
Cytiva's solutions include a variety of empty columns. Heparin Sepharose 6 Fast Flow is compatible with gravity columns, Tricorn columns, XK columns and HiScale columns.
Compatible empty columns – bioprocess scale
For process-scale applications, Cytiva recommends AxiChrom columns. Heparin Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.
Prepacked Heparin Sepharose Fast Flow columns – research scale
Heparin Sepharose Fast Flow resin is available in the following prepacked formats:
HiTrap Heparin Sepharose 6 FF: easy to use with a syringe, a peristaltic pump, or a chromatography system
HiPrep Heparin FF: for convenient scale-up
Prepacked Heparin Sepharose Fast Flow columns – bioprocess scale
Prepacked ReadyToProcess columns are available on request. They eliminate the need for column packing and cleaning validation.
Other Cytiva products for affinity purification of proteins
Looking for a different purification solution? Browse Cytiva's resins and prepacked columns for affinity chromatography or let us help you find the right affinity product.

Specifications

General

Chromatography technique	Group specific affinity
Autoclavability	30 min at 121°C in 0.02 M NaH₂PO₄, pH 7, 5 cycles
BioProcess resin	Yes
Certificate of Analysis	Yes
Chemical stability	Stable in commonly used aqueous buffers - 0.1 M NaOH, 0.05 M sodium acetate, 8 M urea, 6 M guanidine hydrochloride, non-ionic detergents, 10% glycerol
Ligand	Porcine heparin
Ligand Concentration	~3.5 mg heparin/mL resin
Ligand Coupling Method	Reductive Amination
Matrix	cross-linked agarose, 6%, spherical
Particle size, d 50V	~90 μm
Pressure/flow characteristics	250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20oC using buffers with the same viscosity as water)
Storage	4 to 30°C, 20% Ethanol, 0.05 M Sodium Acetate
pH stability, CIP	4–13
pH stability, operational	4–12

FAQs

Question: What is included in the 5 L presentation?

Answer:It contains 5 L of preswollen bulk Heparin Sepharose 6 Fast Flow resin for packing appropriate chromatography columns.

Question: Which molecules can interact with the immobilized heparin?

Answer:Antithrombin and other heparin-binding proteins can be retained, depending on buffer pH, ionic strength and sample composition.

Question: How is retained material typically eluted?

Answer:An increase in salt concentration, applied as a step or gradient, is commonly used to weaken binding and elute target proteins.

Application Note

Cytiva Heparin Sepharose 6 Fast Flow in a 5 L bulk presentation supports larger-scale purification of heparin-binding biomolecules. Porcine heparin ligands are immobilized on cross-linked 6% agarose beads, combining affinity interactions with charge-dependent retention of selected proteins. The resin is particularly relevant when processing heparin-binding plasma proteins and other biomolecules that can be captured reproducibly under controlled buffer conditions. Its preswollen format enables packing into appropriately sized chromatography columns after replacement of the ethanol-containing storage solution. Establish target-specific loading capacity, linear flow, bed geometry and pressure limits before moving from a smaller column to process scale. Salt-gradient or step elution can be used to evaluate recovery and resolution. The medium is delivered in 20% ethanol with sodium acetate as preservative and is not a prepacked column. Use validated cleaning and sanitization procedures so the heparin ligand remains functional across repeated cycles.

Ordering

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Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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