Answer:Strong anion exchange using quaternary ammonium functional groups.
Answer:Q Sepharose High Performance, with an average bead size of about 34 µm.
Answer:Five prepacked columns, each with a 5 mL bed volume.
The Cytiva HiTrap Q HP prepacked chromatography columns contain Q Sepharose High Performance strong anion-exchange resin for laboratory protein purification. Quaternary ammonium functional groups remain positively charged across the applicable operating pH range and bind negatively charged biomolecules under suitable buffer conditions. The high-performance cross-linked agarose media have an average particle size of approximately 34 µm, enabling high-resolution ion-exchange separations. Each column provides a 5 mL packed bed in a convenient HiTrap format suitable for compatible chromatography systems or manual workflows. The pack includes five individual 5 mL columns for purification, sample fractionation and chromatographic method development.
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