Cytiva, 17371703, Capto S ImpAct, 1 L

Name: Capto S ImpAct, 1 L
Brand: Cytiva
SKU: 17371703

A strong cation exchanger for high-resolution polishing of monoclonal antibodies (mAbs) and other biomolecules.

Catalog Number: 17371703

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Category

Ion Exchange Resins

Pack Size1 L

TagsChromatography resins Ion exchange resins Chromatography products resin

CollectionsCytiva Chromatography Columns, Resins & Lab Glassware Cytiva Protein Analysis Equipment & Supplies Collection

Description

Capto™ S ImpAct chromatography resin is a strong cation exchanger designed for polishing of monoclonal antibodies (mAbs), antibody variants, and a wide range of other biomolecules.
High binding capacity, typically > 100 mg mAb/mL resin.
Efficient aggregate removal at high load of mAbs.
High-resolution polishing.
Flexibility of design: large operational window of flow rates and bed heights for easy optimization and scaling.
High productivity, enabling cost-efficient manufacturing.
Capto™ S ImpAct resin is a strong cation exchange resin that is designed for purification of antibodies including bispecific antibodies and antibody fragments. At high load it has shown capabilities of removing aggregates, host cell proteins, and product-related impurities such as charge variants while achieving high recoveries of the target molecule. If you want to evaluate Capto™ S ImpAct resin for developing your antibody process, contact us to request a sample .
A resin designed for polishing of mAbs and antibody variants in GMP environments
The properties of Capto™ S ImpAct enable a highly efficient chromatography step for polishing purification of monoclonal antibodies and antibody variants including bispecific antibodies and antibody fragments. As a second or third chromatography step it can remove impurities such as aggregates, host cell proteins, and product-related impurities such as charge variants or fragments in bind and elute mode. Effective impurity removal and high recoveries can be achieved with loads > 50 mg/mL.
Capto™ S ImpAct is a bioprocess chromatography resin manufactured with validated methods for use in biopharmaceutical production processes. Regulatory support files are available to assist process validation and submissions for regulatory authorities. The manufacturing plant of Capto™ S ImpAct resin is certified for business continuity management (ISO 22301) for continuous reliable supply.
Capto™ S ImpAct resin combines high flow rate, high resolution, and high binding capacity
Capto™ S ImpAct resin includes a polymer-grafted ligand with negatively charged sulfonate and neutral pyrrolidone building blocks. The combination of the groups creates a functionalized surface extender with high binding capacity. The base matrix is based on 50 µm bead size high-flow agarose with optimized pores to give high resolution. The rigid base matrix enables seamless scale up to a reliable and robust chromatography step for large-scale industrialized purification processes.
Capto™ S ImpAct resin is available in the following formats (Fig 1):
Fig 1. Prepacked chromatography formats available from Cytiva to support various scales, from screening to GMP manufacturing.

Specifications

General

Chromatography technique	Ion Exchange Chromatography
Avoid Using	Oxidizing agents, cationic detergent
Binding Capacity/ml Chromatography Medium	>85 mg BSA/mL resin, >90 mg lysozyme/mL resin, >100 mg IgG/ mL resin
BioProcess resin	Yes
Chemical stability	Commonly used aqueous buffers, 1 M sodium hydroxide, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol and 70% ethanol
Estimated shelf life from manufacture date	5 years
Flow velocity	At least 220 cm/h in a 1 m diameter column with bed height 20 cm at 20°C; measured using process buffers with the same viscosity as water at < 3 bar (0.3 MPa)
Ionic Capacity	41 to 60 µmol (H + )/mL medium
Ligand	-SO₃ -
Matrix	High-flow agarose
Particle size, d 50V	~50 μm
Storage	0.2 M sodium acetate in 20% ethanol at 4°C to 30°C
pH stability, CIP	3–14
pH stability, operational	4–12

FAQs

Question: What separation mode does Capto S ImpAct use?

Answer:Strong cation exchange chromatography, using negatively charged ligands to bind appropriately charged proteins.

Question: What is the supplied pack size?

Answer:One liter of bulk chromatography resin for packing a suitable column.

Question: How are bound proteins usually eluted?

Answer:Elution can be achieved by raising salt concentration or adjusting buffer conditions, with the method optimized for the target protein.

Application Note

Cytiva Capto S ImpAct is a strong cation exchange resin supplied as a 1 L package of loose chromatography medium for preparative protein purification. Its negatively charged sulfonate functional groups bind suitable positively charged biomolecules under conditions selected for the target's isoelectric point and buffer composition. The resin supports capture or intermediate purification steps where resolution, process throughput and impurity removal are important. Develop a method by selecting loading pH and conductivity, evaluating breakthrough capacity, and eluting bound material with an appropriate salt gradient or step. Because binding depends on the sample, neither an individual protein's capacity nor recovery should be assumed from resin family data alone. Pack the resin into compatible process hardware and check bed consolidation, pressure–flow characteristics and separation efficiency before production use. Establish cleaning-in-place and storage procedures that preserve resin lifetime and meet process requirements. This product is bulk resin, not a ready-to-use prepacked column.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
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