Cytiva, 17547401, MabSelect SuRe LX protein A resin, 25 mL

Name: MabSelect SuRe LX protein A resin, 25 mL
Brand: Cytiva
SKU: 17547401

A protein A affinity resin developed for high titer antibody processes

Catalog Number: 17547401

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Category

Affinity Tag Purification Resins

Pack Size25 mL

TagsChromatography resins Chromatography products Affinity antibody resin

CollectionsCytiva Chromatography Columns, Resins & Lab Glassware Cytiva Protein Analysis Equipment & Supplies Collection

Description

MabSelect SuRe™ LX is a protein A affinity resin with very high dynamic binding capacity at extended residence times. The chromatography resin is developed for processing of monoclonal antibodies at high titers.
Novel, alkali-tolerant rProtein A ligand withstands rigorous clean-in-place and sanitization procedures with 0.1 to 0.5 M NaOH.
High dynamic binding capacity (DBC) for high titer cultures reduces process time and amount of resin used.
Fulfills industrial demands for security of supply , robust performance, and regulatory support .
Excellent for antibody purification in bioprocess applications
Alkali tolerance, high capacity and low ligand leakage in combination with the rigid base matrix makes MabSelect SuRe LX an excellent choice for purification of monoclonal antibodies for clinical applications.
Part of the MabSelect protein A resin family
MabSelect SuRe LX is a member of the MabSelect family of protein A resins for the capture of monoclonal antibodies at process scale. MabSelect SuRe LX shares features and benefits of MabSelect and MabSelect SuRe such as a rigid, high-flow agarose matrix that ensures excellent pressure/flow properties, low non-specific binding that leads to low impurity levels in the eluate pool, and an oriented ligand coupling for excellent binding capacity. It also features the same alkali-tolerant rProtein A ligand as MabSelect SuRe. This protein ligand provides greater stability than conventional protein A-based resins in the alkaline conditions used in cleaning-in-place (CIP) and sanitization protocols.
Enhanced stability of the protein ligand
The enhanced alkali stability of MabSelect SuRe LX improves process economy and product quality; cleaning can be performed with cost-effective reagents such as sodium hydroxide, eliminating the need for expensive and hazardous cleaning agents such as Gua-HCl. Process economy is also improved by the increased dynamic binding capacity of MabSelect SuRe LX.
Just as for MabSelect SuRe, the novel ligand construct of MabSelect SuRe LX has been proven to have an increased stability towards proteases. Therefore, the level of leakage of the MabSelect SuRe LX ligand during elution is low.
Purification platform design advantage
MabSelect SuRe has been demonstrated to give generic elution conditions for different monoclonal antibodies, which can be applied also to MabSelect SuRe LX as it utilizes the same ligand. This is an advantage when designing generic purification platform processes. The feature is also correlated to the construction of the MabSelect SuRe protein ligand.
Prepacked columns and predictor filter plates.
MabSelect SuRe LX is available in the following formats:
Process development scale – prepacked columns and plates
PreDictor MabSelect SuRe LX – 96-well plates for high-throughput screening or process development.
PreDictor RoboColumn MabSelect Sure LX – miniaturized column format for high-throughput process development.
HiScreen MabSelect SuRe LX columns – optimized for method and process development.
Lab/research scale – prepacked columns for manual protein purification
HiTrap MabSelect SuRe LX chromatography columns – for easy use with a syringe, peristaltic pump, or chromatography system.
Lab/research scale – prepacked columns for automated protein purification and analysis
HiTrap MabSelect SuRe LX chromatography columns – for easy use with a syringe, peristaltic pump, or chromatography system.
Process scale protein purification – prepacked columns
ReadyToProcess MabSelect SuRe LX columns – 1 L and larger chromatography columns for clinical manufacturing that avoid the need for cleaning and cleaning validation.
For samples, contact your local sales representative or customer support .
MabSelect SuRe LX protein A resin builds on the proven track record of MabSelect and MabSelect SuRe resins in downstream processing of monoclonal antibodies.
Learn about prepacked HiScreen MabSelect SuRe LX , ReadyToProcess MabSelect SuRe LX columns , PreDictor MabSelect SuRe LX or PreDictor Robocolumns MabSelect SuRe LX

Specifications

General

Chromatography technique	Antibody affinity chromatography
Binding Capacity/ml Chromatography Medium	Approximately 60 mg human IgG/mL resin.
BioProcess resin	Yes
Certificate of Analysis	Yes
Chemical stability	Stable in all aqueous buffers commonly used in Protein A Chromatography
Ligand	Alkali-stabilized Protein A-derived ( E. coli )
Ligand Coupling Method	Epoxy activation
Matrix	Highly cross-linked agarose
Particle size, d 50V	~85 µm
Pressure/Flow Specification	500 cm/h, AxiChrom 300, bed height 20 cm, operating pressure < 2 bar
Storage	2 to 8°C, 20% Ethanol
pH stability, CIP	2–13.7
pH stability, operational	3–12

FAQs

Question: What does MabSelect SuRe LX capture?

Answer:Its Protein A ligand binds suitable IgG and Fc-containing antibodies for affinity chromatography.

Question: What is the supplied quantity?

Answer:The package contains 25 mL of bulk chromatography resin.

Question: Can the resin be cleaned with sodium hydroxide?

Answer:Yes. The alkali-stabilized ligand supports cleaning-in-place with 0.1–0.5 M sodium hydroxide under validated conditions.

Application Note

MabSelect SuRe LX is an agarose-based Protein A affinity resin for capturing immunoglobulin G and compatible Fc-containing antibodies from clarified biological feed streams. This 25 mL quantity is suited to laboratory column packing, early process screening and small-scale purification development. The alkali-stabilized recombinant Protein A ligand binds accessible antibody Fc regions during loading, allowing nonbinding host-cell components to be washed away before controlled acidic elution. Its rigid, highly cross-linked agarose matrix supports chromatography operation, while the high binding capacity reduces the resin volume required for concentrated antibody feeds. The medium tolerates cleaning-in-place solutions containing 0.1–0.5 M sodium hydroxide under suitable validated protocols. Establish loading residence time, feed conductivity and elution pH using the target antibody rather than applying a universal method. The resin is supplied as bulk chromatography medium, not as a prepacked column or a complete purification kit.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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