Description
Anion exchange chromatography columns The small column volume of 4.7 mL and bed height of 10 cm make HiScreen columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, because the 10 cm bed height provides sufficient residence time. The results can then serve as a basis for linear process scale-up. If necessary, two columns can easily be connected in series to give a bed height of 20 cm. Capto Q resin characteristics Cytiva's resin couples a quaternary ammonium (Q) strong anion exchanger to a chemically modified, high-flow agarose matrix. The rigid agarose matrix provides greater particle rigidity without compromising pore size, which allows for high bed volume and purification of viscous samples at high flow rates. Capto Q resin for scale up High dynamic binding capacity is achieved with a dextran surface extender that coats the agarose matrix. The resin’s high binding-capacity, high flow rate, and low back pressure reduce process cycle times and improve productivity at process scale. Successful use in published research Capto Q resin, which is packed into HiScreen columns, is used in a variety of research applications, including: Development of a malaria vaccine based on the polymorphic block 2 region of MSP-1 Analysis of the proteolysis of bioactive peptides using a peptidomics approach Downsizing a pullulanase to a small molecule in E. coli Other Capto Q formats Capto Q anion exchange resin is also available as bulk resin and in other prepacked formats. Therefore, it possible to use the same resin for development work, pilot studies, and routine production. Other Cytiva ion exchange products Among Cytiva's solutions are a variety of ion exchange chromatography resins, including, Capto, Sepharose, and SOURCE.
