Description
System benefits The broad synthesis scale and optimized hold-up volume combined with a small system footprint enable a broad and flexible use for research and development requirements. Flexible and easy method editing, and the interactive user interface offer ease of use and support scale-up requirements. Robust process control is supported by sensors measuring pressure, air, temperature, UV, and conductivity. Input from external modules is also possible. The ÄKTA oligosynt’s open front ensures easy access to all modules and full ventilation. The flow path is optimized for achieving a low hold-up volume, limiting the dilution of expensive amidites and excessive use of reagents and solvents. Modules included with the system include two high-precision pumps, a pressure monitor, two solvent valves, two amidite valves, a recirculation valve, a column valve, a waste valve, an I/O-box, a UV monitor, and a conductivity monitor. Components of the oligonucleotide synthesis platform Software The ÄKTA oligosynt™ synthesizer is controlled by UNICORN™ software , which provides real-time control of the system. Basic methods for the desired target sequence can be created in minutes using pre-defined methods and the sequence editor. UNICORN™ software also supports evaluation of results. The method overview is transparent with limited use of macros. Columns Cytiva offers a range of columns for oligonucleotide synthesis: Product code Name/description Scale at 350 μmol/g 18110110 Small stainless steel column 1.2 mL* † 10–50 μmol 18110113 Small stainless steel column 6.3 mL* 0.2 mmol 18110116 Small stainless steel column 12 mL* 0.5 mmol 18110119 Small stainless steel column 24 mL* 0.9 mmol 18110122 Small stainless steel column 48 mL* 1.9 mmol 28946841 FineLINE™ 35 oligo column 1.1 – 3.0 mmol 29358075 AxiTide™ 50 column 2.3 – 6.1 mmol 18115298 FineLINE™ 70‡ column 4.5–12 mmol 4.5 – 12 mmol FineLINE™ and AxiTide™ columns have a flexibility in bed heights. Bed heights between 3 and 8 cm have been used for this table. * used with column holder 18113845 † 10 μmol scale using lower loaded support ‡ Used with PFR O-rings and 10 μm filters ordered separately Primer Support™ 5G solid support The ÄKTA oligosynt™ synthesizer and compatible Cytiva columns are designed for use with Primer Support™ 5G polystyrene solid support. This high-loaded solid support for DNA or RNA oligonucleotide synthesis is available using standard DNA and RNA bases as well as with UnyLinker™ solid support as a starting point, making the solid support more flexible for DNA synthesis.
