Cytiva, SH31027.07, HyClone™ Cell Boost™ 7b supplement, powder, 1 L bottle

Cytiva HyClone Cell Boost 7b is a concentrated, chemically defined, animal-derived component-free powder supplement for fed-batch Chinese hamster ovary (CHO) cell culture. The package yields 1 L of supplement solution after reconstitution; the listed volume is not a bottle of premixed liquid. Cell Boost 7b supplies an alkaline amino acid-rich feed component used alongside Cell Boost 7a to support culture performance and recombinant protein yields. The two feed components are dosed as separate solutions because premixing can cause precipitation. A commonly used starting ratio of Cell Boost 7a to 7b is 10:1 by volume, with the actual additions optimized for each cell line and process. The material is a feed supplement, not a complete basal growth medium. Feeding strategy development should track viable cell density, metabolite profiles, osmolality and target-protein quality to establish suitable feed timing and volume.