Answer:It is developed for recombinant protein production using Chinese hamster ovary (CHO) cells in batch or fed-batch culture.
Answer:It specifies the final liquid medium volume after the powder is reconstituted, not a 25 L container of ready-to-use liquid.
Answer:ActiPro is the basal production medium, while Cell Boost 7b is a concentrated nutrient feed intended to supplement compatible fed-batch cultures.
Cytiva HyClone ActiPro is a chemically defined, animal-derived component-free medium developed for Chinese hamster ovary (CHO) cell cultivation and recombinant protein production. The powder quantity is intended to prepare 25 L of final medium after reconstitution, making this format suitable for standardized media preparation during process development and production support. ActiPro can be used in batch and fed-batch CHO workflows where cell growth, productivity and consistency are important. It is designed to function with a defined nutrient-feeding strategy, including the related Cell Boost 7a and 7b supplements where appropriate. Preparation requires controlled water quality, mixing, pH adjustment and filtration or other handling steps specified by the process protocol. Cell-line-specific growth, osmolality, feed volumes and product quality attributes need to be established during process development. This is a complete basal production medium in powder format, not the more concentrated feed supplement or a ready-to-use sterile liquid medium.
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