Description
Cross flow filtration for intermediate scale-up steps Cross flow filtration (CFF), also known as tangential flow filtration (TFF), is a fast and efficient technique for concentration, diafiltration (buffer exchange and desalting), and fractionation of biomolecules. To bridge the several steps between research and production volumes, Cytiva offers a full range of pilot-scale ultrafiltration and microfiltration hollow fiber cartridge filters. Both 30 cm and 60 cm flow path lengths are offered with cartridges that provide an order-of-magnitude membrane area span from 0.12 to 1.15 m² (1.3 to 12.5 ft²). Expert tip for membrane productivity during process scale-up Membrane productivity (flux) is proportional to the processing temperature. To achieve similar productivity between cold room operations and room temperature trials, the cold room configuration should incorporate three to four times the membrane area used in room temperature trials. How to identify and order the right ultrafiltration cartridge filter for your application Cytiva cartridges are produced in a range of housing sizes to suit different applications and process scales. To identify the proper cross flow cartridge filter, you must understand the model numbering convention. Each group of numbers or letters in the model number represents information about the cartridge.
