Lonza, S50-642, Tris Buffer 50 mM
50 mM Tris Buffer, 30 mL
Catalog Number: S50-642
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Pack Size1 vial
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Answer:It contains 50 mM Tris buffer in a 30 mL vial.
Answer:It helps bring acidic or alkaline samples into an appropriate pH range for endotoxin testing.
Answer:The buffer contains less than 0.005 EU/mL endotoxin.
Lonza 50 mM Tris buffer is an endotoxin-controlled sample diluent for analytical preparation before bacterial endotoxin testing. The 30 mL vial provides a buffered alternative to water when samples are excessively acidic or alkaline. Endotoxin assay mixtures generally require a suitable final pH, commonly between pH 6 and 8, to avoid loss of assay responsiveness. Tris buffer can help establish that range during a documented dilution procedure, but the matrix and dilution factor still require evaluation with positive product controls. The specified endotoxin content is below 0.005 EU/mL, reducing the risk of introducing an appreciable blank contribution during low-level measurements. The solution is suitable for preparation of samples used in Lonza endotoxin detection assays. Keep the vial at 2–8 °C, avoid contact with non-endotoxin-controlled equipment, and do not assume pH adjustment alone removes other inhibitors. This is a sample-preparation buffer, not an LAL reagent or endotoxin calibration standard.
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