Merck, 01051619-RNA-5UG, 1301 Cell Line human

Application
Internal control cell in telomere measurements performed by flow cytometry.
Biochem/physiol Actions
1301 is a sub-line of CCRF-CEM, Sigma catalogue no. 85112105. It has unusually long telomeres, useful in tests where telomere length is determined, where it can be used as control cells with known telomere length. This cell line has been recommended as a control cell line for use with the Telomere PNA Kit/FITC for flow cytometry from DakoCytomation.
Human T-cell leukaemia
Preparation Note
RPMI 1640 (R0883) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)
Maintain cultures between 3-9 x 100,000 cells/ml; 37°C; 5% CO₂.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].