Merck, 01818-5G, Acylase I from Aspergillus melleus

General description
Acylase I belongs to the aminoacylase family of enzymes.
Application
Acylase I from Aspergillus melleus has been used to catalyze the Mannich reaction.
Biochem/physiol Actions
Acylase I plays an important role in the amino acid metabolism of organisms. It also plays a role in the acylation of primary and secondary alcohols. Acylase I is involved in alcoholysis.
Analysis Note
Enzyme activity: the optimum temperature is 40-45°C, the optimum pH is 8.0 (stable form pH 6-10). The enzyme is activated by CoCl₂ in the range of 10⁻⁴ to 10⁻³ M.
Other Notes
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol N-acetyl-L-methionine per minute at pH 8.0 and 37°C
Resolution of acetyl amino acids