Merck, 02043018-1VL, 1D10(AAT) Clone 8D10

Immunogen
Various peptides between 9 and 11 amino acids attached to PPD via a terminal cysteine residue
Application
ELISA, lateral flow tests, western blot analysis
Biochem/physiol Actions
Mouse Sp2/0 x Mouse BALB/c spleen
The antibody is directed against α1-antitrypsin (also known as α1-AT or AAT).
Preparation Note
RPMI 1640 + 2mM glutamine + 15% Foetal Bovine Serum (FBS)
Split cells 1:2, an approximate seeding density of 5 x 10,000 cells/cm²; 5% CO₂; 37°C. Please note: cells are loosely adherent, knock the flask to release attached cells. Treat as an attached cell line.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].