Description
General description
Tumor necrosis factor-alpha (TNF-α) is a pro-inflammatory cytokine primarily produced by activated macrophages and T cells, playing a central role in the immune response. It is crucial for the regulation of inflammation, apoptosis, and the activation of other immune cells, facilitating the body′s defense against infections and tumors. However, excessive or dysregulated TNF-α production is associated with various inflammatory and autoimmune diseases, such as rheumatoid arthritis and Crohn′s disease, making it a significant target for therapeutic interventions.
Application
Inflammatory Disease / Immune Response / Autoimmune Disease
Features and Benefits
Ultra-Sensitive Detection: Utilizing SMC®technology, this assay detects TNFα at femtogram-level concentrations, enabling precise quantification in human plasma and serum—even in low-expression conditions.
Familiar Workflow, Elevated Performance: Follows a standard sandwich immunoassay protocol with a unique elution step and digital readout, delivering high signal-to-noise ratios and low background interference.
Expert Scientific Support: Backed by Field Application Scientists and technical support teams experienced in academic, pharma, CRO, and regulated lab environments.
Automation-Ready Workflow: Compatible with liquid handling systems for hands-free operation, reducing variability and increasing throughput.
Packaging
96 well assay
Analysis Note
Sensitivity: LLOQ 200 fg/mL* (SMCxPRO® Instrument)
Standard Curve Range: 50-50,000 fg/mL
Intra-assay CV: Inter-assay CV: Linearity: Serum: 113% Plasma: 105%
Recovery: Serum: 95% Plasma: 83%
*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.
Other Notes
Although TNFα was detectable in non-human primate, rat, feline, canine and equine samples, the assay has not been verified for these species.
Legal Information
FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
