Merck, 03-0182-00, SMC® Human IL-12p70 High Sensitivity Immunoassay Kit

General description
Interleukin-12p70 (IL-12p70) is a heterodimeric cytokine composed of two subunits, IL-12p35 and IL-12p40, and is crucial for the differentiation of T cells into Th1 cells. It plays a significant role in enhancing the immune response by promoting the production of interferon-gamma (IFN-γ) from T and NK cells, thereby enhancing the cytotoxic activity against pathogens and tumors. IL-12p70 is also involved in the regulation of various immune functions and has potential therapeutic implications in cancer and infectious diseases.
Application
Inflammatory Disease / Immune Response / Autoimmune Disease
Features and Benefits
Enables detection of low-abundance IL-12p70: Enables quantification of low-abundance IL-12p70, critical for studying early immune activation, Th1 polarization, and cytokine signaling in infection and autoimmunity.
Specificity for Biologically Active IL-12p70: Targets the heterodimeric p70 form of IL-12, not the p40 subunit or p35 alone. This provides accurate measurement of the functional cytokine, supporting mechanistic studies of T cell differentiation and IFN-γ induction.
Application in Autoimmune and Inflammatory Disease Research: Enables profiling of immune dysregulation and therapeutic response, especially in chronic inflammatory conditions.
Supports Cancer Immunotherapy and Infectious Disease Studies: Facilitates evaluation of immune activation and cytokine feedback loops in tumor microenvironments and pathogen defense.

Packaging
96 well assay
Analysis Note
Sensitivity: LLOQ 100 fg/mL* (SMCxPRO® Instrument)
Standard Curve Range: 50-50,000 fg/mL
Intra-assay CV: Inter-assay CV: Linearity: Serum: 98% Plasma: 103%
Recovery: Serum: 103% Plasma: 97%
*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.
Other Notes
Although IL-12p70 was detectable in mouse, rat, canine, feline and monkey samples, the assay has not been verified for these species.
Legal Information
FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.