Description
General description
TAR DNA-binding protein 43 (TDP-43) plays a crucial role in regulating gene expression, RNA processing, and the transport of RNA within neurons. It is involved in maintaining neuronal health by facilitating the splicing of pre-mRNA and ensuring the stability of mRNA, which is essential for proper protein synthesis. When TDP-43 mislocalizes from the nucleus to the cytoplasm, it forms toxic aggregates that disrupt cellular function and contribute to neuronal degeneration. TDP-43 has become associated with conditions such as amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) as its abnormal accumulation often correlates with disease severity and progression. Investigation into the role of TDP-43 in neuroinflammation and neurodegeneration can help identify potential therapeutic targets that counteract its harmful effects on neuronal health and function in various neurodegenerative disorders.
Application
Alzheimer′s Disease / Aging / Nervous System / Neurodegenerative Diseases / Neurofilament & Neuron Metabolism / Neuronal & Glial Markers / Neurodegenerative Medicine / Neuroscience
Features and Benefits
Ultra-Sensitive Measurement: Detects TDP-43 down to fg/mL levels in serum, plasma and CSF.
Reliable Data: Enhanced dynamic range with improved signal-to-noise ratio ensures accurate and dependable results.
Easy to Adopt: Familiar sandwich immunoassay format allows researchers with ELISA experience to quickly adopt the technology into their workflow.
Automated Workflow: Compatible with automated liquid handling systems, to reduce manual steps and boosts laboratory productivity.
Expert Support: Technical assistance is provided by specialized field scientists and technical teams.
Packaging
96 well assay
Analysis Note
Sensitivity: LLOQ 5,210 fg/mL* (SMCxPRO® Instrument)
Standard Curve Range: 2,600-6,000,000 fg/mL
Intra-assay CV: Inter-assay CV: Linearity: Serum: 105% Plasma 103% CSF: 103%
Recovery: Serum: 96% Plasma: 100% CSF: 89%
*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.
Other Notes
Although TDP-43 was detectable in non-human primate (rhesus macaque and cynomolgus monkey), feline, and canine samples, the assay has not been verified for these species.
Legal Information
FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
