Merck, 03-0207-00, SMC® Human PD-1 High Sensitivity Immunoassay Kit

General description
Programmed cell death protein 1 (PD-1), CD279, is a type I membrane protein found in T cells, B cells, and macrophages that binds Programmed cell death ligand 1 and 2 (PD-L1 and PD-L2). PD-1 is found in both soluble and insoluble forms. PD-1 regulates the body’s immune response through a variety of pathways, down-regulating immune system activity and suppressing T-cell inflammatory activity. PD-1 has been implicated as playing a role in cancers, autoimmune diseases, the immune response to viruses, and in immunological pathways related to brain repair. As an immune checkpoint, PD-1 promotes apoptosis of antigen-specific T-cells in lymph nodes and reduces apoptosis in regulatory T-cells. Blockers of the PD-1 pathway have been approved as cancer therapeutics.
Application
Immuno-Oncology
Features and Benefits
Next-Gen Sensitivity For Low-Expression Targets: Designed to quantify PD-1 even in samples with minimal immune checkpoint activity.
Illuminate Immune Exhaustion: Quantify PD-1 expression on CD8+ T cells to study T-cell exhaustion in cancer and chronic infections.
Advance Immuno-Oncology Research: Track soluble PD-1 as a biomarker of immune suppression, tumor progression, or therapeutic response.
Support Autoimmune and Viral Studies: Investigate PD-1’s role in immune regulation, IL-10 induction, and CD4+ T-cell inhibition.

Packaging
96 well assay
Analysis Note
Sensitivity: LLOQ 3,470 fg/mL* (SMCxPRO® Instrument)
Standard Curve Range: 870-3,000,000 fg/mL
Intra-assay CV: Inter-assay CV: Linearity: Serum: 107% Plasma: 83%
Recovery: Serum: 93% Plasma 83%
*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.
Other Notes
Although PD-1 was detectable in rat, mouse, monkey, and canine samples, the assay has not been verified for these species.
Legal Information
FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.