Description
General description
Programmed cell death protein ligand 1 (PD-L1), also known as cluster of differentiation 274 (CD274) and B7-H1, is a type 1 membrane protein of 290 amino acids. It is found in a variety of cell types and binds programmed cell death protein (PD-1). It has been indicated as playing a role in autoimmune disease, allograft responses, pregnancy, and other disease states. When PD-L1 binds to PD-1, an inhibitory signal is transmitted that ultimately reduces the proliferation of antigen-specific T cells and reduces the rate of apoptosis in regulatory T-cells. By doing so, PD-L1 contributes to the immune evasion of cancer tissues by suppressing the adaptive immune system. Blocking this activity is thought to restore anti-tumor activity. High PD-L1 expression is correlated with poor prognosis with some cancer types. High levels of the soluble form are also associated with poor prognosis and advanced tumor progression. Multiple PD-L1 inhibiting monoclonal antibodies have been approved as cancer therapeutics.
Application
Immuno-Oncology
Features and Benefits
Unlock Hidden Insights in Cancer Immunology: Detect soluble PD-L1 levels associated with tumor progression and poor prognosis, even at sub-picogram concentrations.
Accelerate Biomarker Discovery: Ideal for studying immune checkpoint dynamics in cancer, autoimmune disease, and transplant biology.
Confidence in Every Data Point: High recovery rates and linearity ensure data integrity across sample types. SMC® T
Technology: Digitally detects PD-L1 at ultrasensitive levels—down to 780 fg/mL, ideal for low-expression biomarker studies.
Packaging
96 well assay
Analysis Note
Sensitivity: LLOQ 780 fg/mL* (SMCxPRO® Instrument)
Standard Curve Range: 390-400,000 fg/mL
Intra-assay CV: Inter-assay CV: Linearity: Serum: 120% Plasma: 110%
Recovery: Serum: 117% Plasma: 109%
*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.
Other Notes
Although PD-L1 was detectable in rat, mouse, monkey, and canine samples, the assay has not been verified for these species.
Legal Information
FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
