Merck, 03010040001, PVDF Western Blotting Membranes

General description
PVDF Western Blotting Membranes are hydrophobic, microporous, polyvinylidene difluoride (PVDF) membranes with a pore size of 0.2μm. The PVDF Western Blotting Membranes are ideal for western and dot blots. The high binding capacity, high mechanical strength, and chemical resistance of the PVDF membrane makes it specifically useful for protein blotting. The combination of strong sample retention and low background binding generates excellent signal-to-noise ratios in colorimetric and chemiluminescent detection. The primary binding mechanism includes electrostatic and hydrophobic nature.
Adsorption: Goat IgG: 294μg/cm²; BSA: 131μg/cm²; insulin: 85μg/cm²
Application
Dot/slot blotting
Blotting from 2D gels
Protein sequencing
HIV detection
Hybridoma screening
Cell blotting
High binding capacity, high mechanical strength, and chemical resistance of the PVDF membrane makes itespecially useful for protein blotting.
The combination of strong sample retention with low background binding generates excellent signal-to-noise ratioswith chromogenic and radioactive detection techniques.
The membrane is also ideal for chemiluminescent substrates.

PVDF Western Blotting Membranes has been used in western blot, for transferring proteins for sequencing.
Note: Tested for use with Lumi-Light western blotting substrate or Lumi-LightPlus western blotting substrate.
Analysis Note
Each lot is function tested (dot blot).
Other Notes
For life science research only. Not for use in diagnostic procedures.