Merck, 03285-1KT-F, Cell Counting Kit

General description
Cell Counting kit, also known as FCCK, is a fluorescent cell counting product. The amount of a fluorescent dye, calcein, produced from Calcein-AM (3′,6′-Di(O-acetyl)-2′,7′-bis [N, Nbis-(carboxymethyl) aminomethyl]-fluorescein, tetraacetoxymethyl ester) by esterases in cells is directly proportional to the number of viable cells in a culture medium. Calcein-AM is highly lipophilic due to the presence of acetoxymethyl groups in the molecule, which rapidly permeates into the cell cytoplasm through the membrane.
Application
Cell Counting Kit or FCCK is used for the detection of live cells by fluorometric method.
Features and Benefits
Cell Counting Kit has the following advantages:

• It doesn’t require any radioisotopes to function.
• No solubilization procedure is needed.
• Results are highly reproducible and accurate.