Description
General description
25.69/28.16 kDa (mature/pro-form) calculated.
CD27 antigen (UniProt P41272; also known as CD27, CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7) is encoded by the Cd27 (also known as Tnfrsf7) gene (Gene ID 21940) in murine species. CD27 is a TNF receptor superfamily member that binds to CD70 and mediates the survival, function, and differentiation of T-, B-, natural killer (NK), and plasma cells. Interaction with CD70 provides costimulation in T cells induces signaling events essential for cell proliferation, long-term maintenance of antigen-specific T cells, antiviral responses, antitumor immunity, and alloreactivity. CD27 ligation on B-cells by CD70 causes enhanced plasma cell formation and increased IgG production. CD27 is also used as a marker for memory B cells in the classification of B-cell deficiencies, including common variable immunodeficiency (CVID). CD27 is synthesized with a signal peptide (a.a. 1-23), which was removed posttranslationally to yield the mature type I transmembrane protein with an extracellular (a.a. 24-182) composed of three TNFR-Cys repeats (a.a. 26-63, 64-104, 105-141), followed by a transmembrane region (a.a. 183-203), and a cytoplasmic tail (a.a. 204-250).
Immunogen
Epitope: extracellular domain
Human IgG1 Fc-tagged recombinant mouse CD27 extracellular domain (Sumi, T., et al. (2008). Immunol. Lett. 119(1-2):91-96).
Application
Neurtralizing Analysis: A representative lot, when injected intraperitoneally following OVA-expressing B16 melanoma cells (B16-cOVA) implant, suppressed B16-cOVA lung metastasis and tumor growth among wild-type, but not Cd27-knockout, mice (Roberts, D.J., et al. (2010). J. Immunother. 33(8): 769 779).
Neurtralizing Analysis: A representative lot, when injected intraperitoneally during the induction phase of ragweed-induced experimental allergic conjunctivitis (EC), significantly affected the systemic immune responses, but not the severity of EC in mice (Sumi, T., et al. (2008). Immunol. Lett. 119(1-2):91-96).
Research Category
Inflammation & Immunology
This rat monoclonal Anti-CD27 Antibody, clone RM27-3E5, Cat. No. 04-1475-I is validated for use in Flow Cytometry and Neutralization applications for the analysis of CD27.
Biochem/physiol Actions
Clone RM27-3E5 enhanced anti-CD3-stimulated T-cell proliferation when immobilized in vitro and augmented OVA-specific CTL induction when administrated in vivo (Roberts, D.J., et al. (2010). J. Immunother. 33(8): 769 779; Sumi, T., et al. (2008). Immunol. Lett. 119(1-2):91-96).
Physical form
Format: Purified
Protein G purified.
Purified rat IgG2a in PBS without preservatives.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Flow Cytometry in mouse splenocytes.
Flow Cytometry Analysis: 2 µL of this antibody detected CD27-positive mouse splenocytes.
Other Notes
Concentration: Please refer to lot specific datasheet.
Replaces: 04-1475
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
