Merck, 05-826-AF488, Anti-Bcl-2 Antibody, clone AW604, Alexa Fluor™ 488 Conjugate

General description
Apoptosis regulator Bcl-2 (UniProt P10415) is encoded by the BCL2 gene (Gene ID 596) in human. Bcl-2 is an anti-apoptotic and the founding member of the Bcl-2 family of proteins that play either a pro- or anti-apoptotic role in cell death signaling. Bcl-2 family members are related by regions of sequence and structural homology. The pro-survival members contain four Bcl-2 homology regions (BH1–BH4), whereas the BH3-only members contain only the BH3 amphipathic helix, which mediates their interaction with the groove of multi-domain BCL-2 family members. Human BCL2 gene mutations are well known causes of many cancers, including melanoma, breast, prostate, chronic lymphocytic leukemia, and lung cancer.
Immunogen
KLH-conjugated, synthetic peptide corresponding to amino acids 41-54 (C-GAAPAPGIFSSQPG) of human Bcl-2.
Application
This Anti-Bcl-2 Antibody, clone AW604, Alexa Fluor™ 488 Conjugate is validated for use in Immunocytochemistry, Flow Cytometry for the detection of Bcl-2.

Flow Cytometry Analysis: 1.0 ug of this antibody from a representative lot detected Bc
Analysis Note
Evaluated by Immunocytochemistry in Jurkat cells.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected Bcl-2 in Jurkat cells.
Other Notes
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
Legal Information
ALEXA FLUOR is a trademark of Life Technologies