Merck, 05062302-DNA-5UG, BHK21-InVitrus

Biochem/physiol Actions
BHK21CL13-2P cell line (Sigma Catalogue number. 84111301) adapted to grow in the chemically defined protein- and peptide-free InVitrus™ medium.
Hamster Syrian kidney, serum-free
Preparation Note
InVitrus™ medium + 4mM Glutamine + 5 ug/ml Insulin. When freezing cells down use InVitrus medium + 4mM Glutamine (50:50 conditioned medium:fresh medium) + 10% DMSO.
Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Tran
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].
Legal Information
InVitrus is a trademark of Cell Culture Technologies