Application
Bovine serum albumin has been used to quantify proteins in raw enzyme extracts. It has also been used as a blocking agent for mitochondrial imaging.
Albumin from bovine serum can be used for Electron and Immunoelectron microscopy. It can also be used for isolating and culturing neonatal rat cardiac ventricular myocytes.
Biochem/physiol Actions
Bovine serum albumin also refers as BSA or Fraction V is a protein isolated from cows. BSA can block vacant binding sites in enzyme-linked immunosorbent assay (ELISA) in both poly-l-lysine (PLL)-treated and non-treated microwells. It can also trigger insulin dependent diabetes mellitus.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Preparation Note
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
Other Notes
Review
Sales restrictions may apply

Specifications

General

UNSPSC Code	12352202
EC Number	232-936-2
NACRES	NA.27
MDL number	MFCD00130384
eCl@ss	32160409
biological source	bovine
assay	≥98.0% (GE)
form	lyophilized powder
Quality Level	200
Gene Information	bovine ... ALB(280717)
packaging	glass bottle of
technique(s)	cell culture \| mammalian: suitable
pH	4.8-7.5
solubility	H2O: slightly soluble
anion traces	chloride (Cl-): ≤2000 ppm
cation traces	Ca: ≤500 ppm
UniProt accession no.	P02769
storage temp.	2-8°C

FAQs

Question: What purity does this BSA provide?

Answer:The lyophilized bovine serum albumin has an assay of at least 98% by gel electrophoresis.

Question: What physical form is supplied?

Answer:It is supplied as a crystallized lyophilized powder.

Question: How should it be stored?

Answer:The product is stored at 2–8°C.

Application Note

Bovine Serum Albumin is used as a high-purity protein reagent for cell culture, microscopy, protein assays, blocking, stabilization, and other biochemical workflows. This product is a crystallized lyophilized bovine serum albumin powder with an assay of at least 98% by gel electrophoresis. BSA can serve as a carrier or stabilizing protein, a blocking component in immunochemical procedures, and a protein standard or matrix component in laboratory assays. It is also suitable for mammalian cell-culture and electron-microscopy applications. The dry form supports controlled preparation of stock solutions at the concentration required by a specific protocol. This configuration contains 25 g and is stored at 2–8°C. Its defined purity and broad laboratory utility make it useful when a consistent albumin source is needed across protein, imaging, and cell-based experiments.

Ordering

Notice

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Merck, 05470-25G, Bovine Serum Albumin

Send an RFQ.

General

Question: What purity does this BSA provide?

Question: What physical form is supplied?

Question: How should it be stored?

Notice

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