Description
General description
Interleukin-22 receptor subunit alpha-1 (UniProt Q8N6P7; also known as CRF2-9, Cytokine receptor class-II member 9, Cytokine receptor family 2 member 9, IL-22 receptor subunit alpha-1, IL-22R-alpha-1, IL-22RA1, ZcytoR11) is encoded by the IL22RA1 (also known as IL22R) gene (Gene ID 58985) in human. IL-22RA-1 is a member of the type II cytokine receptor family that complexes with IL-10RB and mediates JAK/STAT signaling. IL22 binding to heterodimer is greater than binding to IL22RA1 alone. IL-22RA-1 is expressed in colon, liver, lung, pancreas and kidney, however, no expression is observed in immune cells, such as monocytes, T-cells, and NK-cells. It is also detected in normal blood brain barrier endothelial cells as well as in multiple sclerosis lesions. Higher expression has been observed in synovial fluid cells from rheumatoid arthritis and spondyloarthropathy patients.
~63 kDa observed. 61.54 kDa (signal peptide a.a. 1-15 removed) calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
KLH-conjugated linear peptide corresponding to a sequence within the C-terminal region of human IL-22RA-1 cytoplasmic domain.
Application
Flow Cytometry Analysis: 1 µg from a representative lot detected IL-22RA-1 immunoreactivity among one million 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized SW480 human colon cancer cells.
Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected IL-22RA-1 in human tonsil and prostate tissue sections.
Research Category
Inflammation & Immunology
This rabbit polyclonal Anti-IL-22RA-1 Antibody, Cat. No. 06-1077-I is validated for use in Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting for the detection of Interleukin-22 receptor subunit alpha-1.
Biochem/physiol Actions
This polyclonal antibody targets a C-terminal sequence within the cytoplasmic domain of human IL-22 receptor subunit alpha-1.
Physical form
Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in mouse spleen tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected IL-22RA-1 in 10 µg of mouse spleen tissue lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Replaces: 06-1077
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
