Merck, 06011805-DNA-5UG, 1411H

Biochem/physiol Actions
A non seminomatous germ cell carcinoma derived cell line. A yolk sac carcinoma cell line derived from a testicular teratomacarcinoma. This cell line is tumourigenic in nude mice.
Human yolk sac carcinoma
Preparation Note
DMEM (High Glucose) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). No sodium pyruvate.
Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 10% CO₂; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].