| UNSPSC Code | 12352202 |
|---|---|
| eCl@ss | 32160405 |
| Web Name Suffix Override | from rat tail, liquid, 2-5 mg/mL, suitable for cell culture, used for 3D gel formation |
| Web Name Override | Rat Collagen Type I |
| NACRES | NA.75 |
| form | liquid |
| biological source | rat |
| manufacturer/tradename | Upstate® |
| concentration | 100 mg |
| technique(s) | cell culture | mammalian: suitable (and for 3D proliferation assays.) |
| input | sample type hematopoietic stem cell(s)sample type mesenchymal stem cell(s)sample type induced pluripotent stem cell(s)sample type epithelial cellssample type pancreatic stem cell(s)sample type neural stem cell(s)sample type: mouse embryonic stem cell(s) |
| NCBI accession no. | NM_000088.3 |
| UniProt accession no. | P02452 |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| Quality Level | 100 |
| Gene Information | rat ... COL1A1(29393) |
Answer:It is type I collagen isolated from rat-tail tendon.
Answer:The product contains 100 mg of collagen as an acidic liquid solution.
Answer:Store at 2–8 °C and do not freeze.
Collagen Type I, rat tail is used for preparing extracellular-matrix coatings and three-dimensional collagen gels in mammalian cell-culture research. The product contains 100 mg of type I collagen isolated from rat-tail tendon, supplied as a liquid in 0.02 N acetic acid. Its concentration is lot-dependent within approximately 2–5 mg/mL, so the total protein amount rather than a fixed solution volume defines this package. Type I collagen supports cell attachment and spreading when applied as a substrate coating and can form gels for three-dimensional cell-proliferation assays. The material has been evaluated for uniform gelation and for attachment and spreading of PC-12 rat cells. Researchers can use the acidic collagen stock in culture-coating preparations or in procedures that form a neutralized collagen matrix for cell experiments. This is a rat-derived extracellular-matrix component, not collagenase or a cell-dissociation reagent. The product ships on wet ice, should be stored at 2–8 °C, and must not be frozen. The stated stability is one year at approximately 4 °C from shipment.
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