Merck, 09978-500G, Ammonium sulfate

General description
Ammonium sulfate is naturally present as a rare mineral mascagnite in volcanic fumaroles and due to coal fires in some dumps.
Application
Ammonium sulfate has been used:
to prepare mobile phase A for hydrophobic interaction chromatography (HIC)
as reference material for the thermal decomposition of NH4NO3 (S1–S6) thermal decomposition to N2O (S1-N2O–S6-N2O)
to prepare the stock solution for M63 medium
as a reagent in molecular biology and chromatography
in the precipitation and fractionation of proteins
in the purification of antibodies
for the crystallographic analysis of nucleic acids and proteins
in high-performance liquid chromatography (HPLC) analysis of proteins, such as in hydrophobic interaction chromatography
for Sepharose chromatography of tRNAs using reverse salt gradients
in long PCR buffer, in PCR lysis solution, and second-strand cDNA synthesis
HPLC analysis of phospholipids

Biochem/physiol Actions
Ammonium sulfate is an inorganic salt, which is mostly used as a soil fertilizer. It is also used as a flame retardant since it elevates the combustion temperature of the material and lowers maximum weight loss rates. This leads to an upsurge in the production of residue or char.
Other Notes
The most commonly used salt for the fractional precipitation of proteins due to its solubility and effectiveness; Protein crystallization; Sepharose™ chromatography of tRNAs using reverse salt gradients.
Legal Information
Sepharose is a trademark of Cytiva