Merck, 1.00482, Periodic acid solution 0.5%

General description
The Periodic acid solution 0.5% for the PAS reaction (periodic acid Schiff) for the detection of aldehyde and mucosubstances in microscopy, is a ready to use solution used for the detection of mucopolysaccharides, glycogen, muco- and glycoproteins, glyco-, phospholipids, basal membranes and collagen. The material is treated with periodic acid creating aldehyde groups which react with Schiff′s reagent and give a bright red color reaction, thus the nuclei appear blue.
In the PAS reaction, the histological specimen material is first treated with periodic acid, resulting in the oxidation of the 1,2-glycols into aldehyde groups. The addition of Schiff′s reagent (fuchsin-sulfuric acid)(Product number 1.09033) in the second step causes the aldehydes to react to form a brilliant red color. In the end result, the PAS reaction yields a specific color reaction with unsubstituted polysaccharides, neutral mucopolysaccharides, muco- and glycoproteins, and glyco- and phospholipids.
The PAS reaction can be further combined with the Alcian blue staining method to detect mucosubstances (glycosaminoglycans).
It is an IVD / CE registered & certified product that can be applied in clinical diagnostics due to its excellent quality. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Analysis Note
Nuclei: blue
polysaccharides: purple
glycogen: purple
neutral mucopolysaccharides: purple
mucoproteins: purple
glycoproteins: purple
glycolipids: purple
phospholipids: purple
Basement membrane: purple
Collagen: purple