Merck, 1.01295, Coliforms 50

General description
Coliforms 50 is a granular, sterile, and ɣ-irradiated microbiological culture medium designed for the detection of coliforms and Escherichia coli in 50 ml water samples.
Application
Coliforms 50 is used for the detection of coliforms and E. coli in water and beverage samples, as well as for microbiological culture testing for environmental and industrial monitoring.
Analysis Note
Appearance
- clearness:
clear
- colour: yellowish
pH-value (25 °C): 6.6 - 7.0
Inoculum on reference medium
- Escherichia coli ATCC 11775 (WDCM 00090):
10 - 100
- Citrobacter freundii ATCC 8090: 10 - 100
- Klebsiella pneumoniae ATCC 31488: 10 - 100
- Salmonella typhimurium ATCC 14028 (WDCM 00031): 10 - 100
- Pseudomonas aeruginosa ATCC 10145 (WDCM 00024): ≥ 1000
Growth
- Escherichia coli ATCC 11775 (WDCM 00090):
+
- Citrobacter freundii ATCC 8090: +
- Klebsiella pneumoniae ATCC 31488: +
- Salmonella typhimurium ATCC 14028 (WDCM 00031): +
colour change to blue-green
- Escherichia coli ATCC 11775 (WDCM 00090):
+
- Citrobacter freundii ATCC 8090: +
- Klebsiella pneumoniae ATCC 31488: +
- Salmonella typhimurium ATCC 14028 (WDCM 00031): -
- Pseudomonas aeruginosa ATCC 10145 (WDCM 00024): -
Fluorescence
- Escherichia coli ATCC 11775 (WDCM 00090):
+
- Citrobacter freundii ATCC 8090: -
- Klebsiella pneumoniae ATCC 31488: -
- Salmonella typhimurium ATCC 14028 (WDCM 00031): -
- Pseudomonas aeruginosa ATCC 10145 (WDCM 00024): -
Indole formation
- Escherichia coli ATCC 11775 (WDCM 00090):
+
- Citrobacter freundii ATCC 8090: -
- Klebsiella pneumoniae ATCC 31488: -
- Salmonella typhimurium ATCC 14028 (WDCM 00031): -
Incubation: 24 hrs.; 35 °C; aerobic.
Legal Information
READYCULT is a registered trademark of Merck KGaA, Darmstadt, Germany