Merck, 1.01350, Leishman′s eosine methylene blue

General description
The dry mixture of the Leishman′s eosin methylene blue dye for microscopy, is used for the self-preparation of the Leishman′s staining solution. The staining method according to Leishman is one of the standard techniques in hematological diagnostic procedures. Like Wright′s eosin methylene blue and May-Grünwald′s eosin methylene blue, it is used for staining of blood and bone marrow smears and clinical cytological specimens. The dye is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Analysis Note
Identity (UV/VIS-Spectrum): passes test
pH-value (0,01 %; methanol 50 %): 5 - 6
Absorption maximum λ 1 (Methanol): 623 - 627 nm
Absorption maximum λ 2 (Methanol): 522 - 525 nm
Spec. Absorptivity A 1%/1cm
- λ1max; 0.0005 %; methanol; calculated on dried substance:
800 - 1100
- λ2max; 0.0005 %; methanol; calculated on dried substance: 650 - 850
TLC-Test: passes test
Loss on drying (105 °C): ≤ 8 %
Suitability for microscopy: passes test