Merck, 1.02137, Contact E

General description
Envirocheck Contact Enterobacteriaceae, a semi-quantitative detection of microorganisms on surfaces and in liquids, are flexible paddles that are coated with Tryptic soy agar to detect the bacteria belonging to the family Enterobacteriaceae from the samples. Slide A is coated with Plate Count Agar to obtain the total viable (aerobic) count. Slide B is coated with Violet Red Bile Dextrose (VRBD) Agar, recommended for the isolation and enumeration of all Enterobacteriaceae species in foodstuffs.
Application
This medium is recommended for the selective isolation and enumeration of all Enterobacteriaceae species in foodstuffs. Envirocheck contact slides can be used for both surface and liquid testing in industrial and environmental monitoring.
Analysis Note
Count the number of colonies on both sides of the paddle. Plate Count Agar: Count the number of colonies for obtaining the total aerobic bacterial count (TVC). Violet Red Bile Dextrose (VRBD) Agar: Degradation of dextrose is accompanied by production of acid which is indicated by a colour change to red and by zones of precipitated bile acids surrounding the colonies.
Sterility: passes test
pH-value/side one (25 °C): 6.7 - 7.3
pH-value/side two (25 °C): 7.0 - 7.6
Growth/side one
- Escherichia coli ATCC 8739 (WDCM 00012):
good
- Staphylococcus aureus ATCC 6538 (WDCM 00032): good
- Salmonella typhimurium ATCC 14028 (WDCM 00031): good
- Shigella sonnei ATCC 25931: good
Growth/side two
- Escherichia coli ATCC 8739 (WDCM 00012):
fair/good
- Staphylococcus aureus ATCC 6538 (WDCM 00032): none
- Salmonella typhimurium ATCC 14028 (WDCM 00031): fair/good
- Shigella sonnei ATCC 25931: fair/good
Incubation: 48 hrs.; 35 °C; aerobic.
Legal Information
ENVIROCHECK is a trademark of Merck KGaA, Darmstadt, Germany