Merck, 1.02147, Contact DC

General description
(Disinfection Control) Envirocheck™
Envirocheck Contact Disinfection Control, a semi-quantitative detection of microorganisms on surfaces and in liquids, are flexible paddles that are coated with Tryptic soy agar to check the effectiveness of disinfection. Slide A is coated with CASO (Tryptic Soy) Agar to determine the total viable (aerobic) count and Slide B is coated with CASO (Tryptic Soy) agar containing neutralizers such as Polysorbat 80, Sodium thiosulphate, Lecithin and Histidine. The use of these neutralizers allows growth of bacteria that were held in bacteriostasis after sanitation/disinfection but still viable
Application
Envirocheck contact slides can be used for both surface and liquid testing.
Analysis Note
Count the number of colonies on both sides of the paddle. CASO Agar: count the number of colonies for obtaining the microbial load before disinfection. CASO Agar with neutralizers: count the number of colonies to obtain the microbial load of surviving microorganisms after disinfection. Growth indicates that disinfection was either not adequate (too low concentration of disinfectant) or the effective time was too short.
Microbial contamination: no growth
pH-value/side one (25 °C): 7.0 - 7.6
pH-value/side two (25 °C): 7.0 - 7.6
Growth/side one
- Escherichia coli ATCC 8739 (WDCM 00012):
good
- Staphylococcus aureus ATCC 6538 (WDCM 00032): good
- Candida albicans ATCC 10231 (WDCM 00054): good
- Bacillus subtilis ATCC 6633 (WDCM 00003): good
- Pseudomonas aeruginosa ATCC 9027 (WDCM 00026): good
- Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053): good
Growth/side two
- Escherichia coli ATCC 8739 (WDCM 00012):
good
- Staphylococcus aureus ATCC 6538 (WDCM 00032): good
- Candida albicans ATCC 10231 (WDCM 00054): good
- Bacillus subtilis ATCC 6633 (WDCM 00003): good
- Pseudomonas aeruginosa ATCC 9027 (WDCM 00026): good
- Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053): good
Incubation: 48 hrs.; 35 °C; aerobic; Aspergillus up to 72 hrs.
Legal Information
ENVIROCHECK is a trademark of Merck KGaA, Darmstadt, Germany