Description
General description
Biological detergent
Agent for reversible denaturation of proteins. Special grade with low optical density for use in procedures where spectrometry is involved.
Application
Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.
Analysis Note
Assay (Perchloric acid titration, calc. on anhydrous substance): ≥ 99.5 %
Identity (IR-spectrum): passes test
pH (100 g/l, water): 5.5 - 6.5
Melting point: 183 - 188 °C
Absorbance A (235 nm; 50 %; 1 cm; water): ≤ 0,2
Heavy metals (as Pb): ≤ 10 ppm
Fe (Iron): ≤ 5 ppm
Sulfated ash (600 °C): ≤ 0.05 %
Water (according to Karl Fischer): ≤ 1