Merck, 1.07627, <I>Pseudomonas </I>CFC Selective Supplement

General description
The Pseudomonas CFC Selective Supplement is a mixture of 3 different inhibitors in lyophilized form. Cetrimide, Fucidin, and Cephalothin inhibit the accompanying Gram-positive and Gram-negative flora.
Application
Pseudomonas CFC Selective Supplement is an additive for the preparation of GranuCult® prime Pseudomonas CFC/CN Agar (Base) (1.07620) for the detection and enumeration of Pseudomonas spp. from food and animal feed.
Analysis Note
Recovery on test medium
- Pseudomonas aeruginosa ATCC 27853 (WDCM 00025):
≥ 70 %
- Pseudomonas putida ATCC 12633 (WDCM 00117): ≥ 70 %
- Pseudomonas fluorescens ATCC 13525 (WDCM 00115): ≥ 70 %
- Pseudomonas fragi ATCC 27362: ≥ 70 %
- Pseudomonas fragi ATCC 4973 (WDCM 00116): ≥ 70 %
- Burkholderia cepacia ATCC 17759: ≥ 70 %
- Proteus mirabilis ATCC 14153: ≤ 0.01 %
- Staphylococcus aureus ATCC 25923 (WDCM 00034): ≤ 0.01 %
Colonies
- Pseudomonas aeruginosa ATCC 27853 (WDCM 00025):
greenish
- Pseudomonas putida ATCC 12633 (WDCM 00117): greenish
- Pseudomonas fluorescens ATCC 13525 (WDCM 00115): greenish
- Pseudomonas fragi ATCC 27362: milky
- Pseudomonas fragi ATCC 4973 (WDCM 00116): milky
- Burkholderia cepacia ATCC 17759: milky
Incubation: 44 hrs.; 25 °C; aerobic;
Reference: blood-agar (24-48 hrs.; 30 °C; aerobic).

Supplement tested in Pseudomonas selektiv agar (base) art. no. 1.07620.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany