Merck, 1.09844, Eosin Y-solution 0.5% aqueous

General description
Eosin Y-solution 0.5% aqueous - for microscopy, is used for human-medical cell diagnosis and serves the histological and clinico-cytological investigation of sample material of human origin. The hematoxylin and eosin (H&E) staining method is the method most frequently used for the staining of histology material. This Eosin Y-solution is a 0.5% aqueous staining solution, that can be used e. g. for the histological staining of instantaneous sections.The staining mechanism behind the H&E staining is a physico-chemical process. In the first step, the positively charged nuclear dye (hematoxylin) Mayer′s hemalum solution for microscopy (Produt number 1.09249) or Hematoxylin solution modified acc. to Gill III (Product number 1.05174) binds to the negatively charged phosphate groups of the nucleic acid of the cell nucleus. The nuclei will be dyed dark blue to dark violet. The second step is the counterstaining with negatively charged anionic Eosin Y, a xanthene dye. Eosin binds to the positively charged plasma proteins. Cytoplasm and intercellular substances are stained pink to red, while erythrocytes will appear with yellow to orange color.
The 500ml bottle provides 1500 stainings. It is an IVD registered product and CE certified, thus can be used for clinical diagnostic purposes. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Analysis Note
Suitability for microscopy (Tissue section): passes test
nuclei: dark blue to dark violet
Cytoplasm: pink to red
intercellular substances: pink to red
Erythrocytes: yellow to orange