Merck, 1.10549, <i>Listeria</i> Enrichment Broth

General description
The Granucult® Listeria Enrichment Broth formulation is a modified Tryptic Soy (CASO) Broth with an additional 6 g/liter of yeast extract. The inhibition of undesired accompanying flora is achieved by the addition of acriflavin HCl, cycloheximide, and nalidixic acid. In contrary to Listeria Enrichment Broth (Base) (1.11951), this broth already contains antibiotic substances.
Application
Granucult® Listeria Enrichment Broth is intended for the selective enrichment of Listeria.
Analysis Note
Appearance (clearness): clear
Appearance (colour): yellowish-brown
pH-value (25 °C): 7.1 - 7.5
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): 10 - 100
Inoculum on reference medium (Listeria monocytogenes ATCC 19114 (WDCM 00110)): 10 - 100
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)): 10 - 100
Inoculum on reference medium (Listeria innocua ATCC 33090 (WDCM 00017)): 10 - 100
Colony count (Listeria monocytogenes ATCC 13932 (WDCM 00021)): ≥ 1E+04 CFU/ml
Colony count (Listeria monocytogenes ATCC 19114 (WDCM 00110)): ≥ 1E+04 CFU/ml
Colony count (Listeria monocytogenes ATCC 35152 (WDCM 00109)): ≥ 1E+04 CFU/ml
Colony count (Listeria innocua ATCC 33090 (WDCM 00017)): ≥ 1E+04 CFU/ml
Colony count (Staphylococcus aureus ATCC 6538 (WDCM 00032)): ≤ 1000 CFU/ml
Incubation: 24 hrs.; 30 °C; aerobically.
Other Notes
Replaces: 1.10549.5007
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany