Merck, 1.14894, Fractogel®EMD SE Hicap (M)

General description Strong cation exchange Fractogel® resin featuring the tentacle technology, for purification of basic and neutral proteins (e.g. antibodies) and peptides Features and Benefits Fractogel® EMD SE Hicap (M) features include: Tentacle technology provides high yield, selectivity, and focused elution High loading capacity combined with high purity at lower elution volumes Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution Packaging 1.14894.0100: Fractogel® EMD SE Hicap (M) Resin 100ml 1.14894.0010: Fractogel® EMD SE Hicap (M) Resin 10ml 1.14894.0500: Fractogel® EMD SE Hicap (M) Resin 500ml 1.14894.5000: Fractogel® EMD SE Hicap (M) Resin 5L Plastic bottle Analysis Note Appearance: Milky turbid suspension,free from impurities(foreign particles) Microscopic evaluation: Uniform spherical particles,no agglomerates,no fines Extractable matter (water): ≤ 0.08 % Cerium: ≤ 20 µg/g Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar Particle size (d10): 37 - 45 µm Particle size (d50): 48 - 60 µm Particle size (d90): 63 - 77 µm Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml Endotoxins: ≤ 1.00 EU/ml Protein binding capacity (lysozyme): 120 - 160 mg/ml Functional test (b:a): ≤ 0.20 Functional test: Separation of chymotrypsinogen A and lysozyme Legal Information FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany