Merck, 1.15973, Weigert′s iron hematoxylin kit

General description
Weigert′s iron hematoxylin kit - for nuclear staining in histology is a ready to use staining kit used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin, for example histological sections of e. g. the liver, the kidney, the intestine, the placenta and similar. Aluminium hematoxylins (e. g. nuclear fast red aluminium sulfate) are washed out of the tissue sections by acidic staining solutions. Iron hematoxylin solutions (e. g. Weigert′s iron hematoxylin), on the other hand, are acid-resistant and are preferentially used for staining nuclei in histological tissue sections in which counterstaining is done with acidic staining solutions. Examples here include the visualization of elastic fibers according to the van Gieson method (e. g. Elastica van Gieson (Product number 1.15974)) and the various trichromo connective-tissue staining methods (e. g. according to Masson-Goldner (Product number 1.00485). Commonly used hemalum solutions such as Mayer′s Hemalum, Delafield, Gill and Harris tend to procedure also weak nucleic staining only when used with acidic counter-stains.The kit contains two solutions; an alcoholic hematoxylin solution and a hydrochloric acid iron(III)nitrate solution, which are ready-to-use and should be applied at 3-5 μm paraffin sections.
The package is sufficient for 400 - 500 applications. Weigert′s Iron hematoxylin kit is an IVD & CE product and can be used in the clinical laboratory for diagnostics. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.