Merck, 1.25058, Fractogel®EMD DEAE (M)

General description 8 x 20mm Features and Benefits MiniChrom Column Fractogel® DEAE (M), 1 ml enables: Excellent binding to large viruses and plasmid DNA Homogenous binding with high selectivity and purity Lower elution volumes for the highest purity levels Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 9.5. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD DEAE when the pH of the buffer is about 1 unit above their isoelectric points (pl). The strength of the binding depends on the following: the buffer system pH value of the buffer which determines the surface charge of the protein the degree of the ionization of the functional groups of the exchanger the concentration of the counter ions the charge density on the support (protein binding capacity) Analysis Note Appearance: passes test Legal Information FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany