Merck, 1.25082, Fractogel®EMD COO^- (M)

General description 8 x 100mm Features and Benefits MiniChrom Column Fractogel® COO (M), 5 ml enables: Good resolution between monomers and aggregates Efficient aggregate and HCP removal combined with high yield Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution Due to the titration behavior, the ion exchange capacity can be used from pH 6 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD COO- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl). The strength of the binding depends on the following: the buffer system pH value of the buffer which determines the surface charge of the protein the degree of the ionization of the functional groups of the exchanger the concentration of the counter ions the charge density on the support (protein binding capacity) Legal Information FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany