Merck, 1.46186, <i>Listeria</i> Agar

General description
ReadyPlate™ Listeria Agar plates are ready-to-use, sterile 90mm plates for the enumeration, detection, and identification of Listeria monocytogenes and other Listeria sp. from food and feeding stuff. This medium contains the chromogenic compound 5-bromo-4- chloro-3-indolyl-β-D glucopyranoside, a substrate for the detection of β-glucosidase. This enzyme is commonly produced by all Listeria sp. that appear on the medium as blue-green colored colonies. Differentiation of Listeria monocytogenes from other Listeria sp. is achieved through the production of a phosphatidylinositol-specific phospholipase C (PI-PLC). Listeria monocytogenes hydrolyses the specific purified substrate added to the medium producing an opaque halo around the colonies.
This basic pH of the medium, ensures good growth for a broad range of bacteria. The addition of inhibitors results in the reduction of the growth of the majority of concomitant Gram-positive and Gram-negative pathogens, as well as of yeasts and fungi. Selectivity is obtained by the addition of lithium chloride, nalidixic acid, and cycloheximide.
Application
Listeria Agar are ready-to-use, sterile 90mm plates for isolation and differentiation of Listeria monocytogenes and other Listeria sp. from food and animal feed, environmental samples in the area of food production, food handlings and other materials.
Legal Information
READYPLATE is a trademark of Merck KGaA, Darmstadt, Germany