Merck, 10102105001, Adenosine Deaminase (ADA)

Name: Adenosine Deaminase (ADA)
Brand: Merck
SKU: 10102105001

Size:2 mL, ADA, from calf intestine

Catalog Number: 10102105001

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Category

ELISA Substrates

Pack Size2 mL

TagsLabware Labware Partners Roche<sup>®</sup> Products

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Adenosine deaminase (ADA) is a zinc metalloenzyme.
Adenosine Deaminase (ADA) is present majorly in the proximal small intestine and the duodenum. It is also called Adenosine aminohydrolase. It is a zinc metalloenzyme and exists as a high (230−440 kDa) and low molecular weight (30−47 kDa) isoforms.
Application
Adenosine deaminase has been used to pre-treat primary Kupffer cells.
Adenosine Deaminase (ADA) has been used as a component of macrophage- serum-free media (SFM) to treat macrophages.
Biochem/physiol Actions
Adenosine deaminase (ADA) is involved in the deamination of adenosine to inosine and 2′ deoxyadenosine to 2′-deoxyinosine.
Adenosine Deaminase (ADA) catalyzes the deamination of adenosine analogs to the corresponding inosine analogs. It is present in high levels in colorectal cancer and is a potential marker in tuberculous pleuriti. ADA deficiency affects T-and B-lymphocytes resulting in severe combined immunodeficiency (SCID) leading to lymphocytopenia.
Physical form
Solution in 50% glycerol (v/v), 10 mM potassium phosphate, pH approximately 6
Preparation Note
Storage conditions (working solution): ADA (all preparations) may be dialyzed, without loss of activity, at 2 to 8 °C against 20 mM phosphate buffer, pH 7. The dialyzed preparation is stable at -15 to -25 °C.
Analysis Note
Contaminants: <0.01% alkaline phosphatase, AMP-deaminase, guanase, and nucleoside phosphorylase each
Other Notes
For life science research only. Not for use in diagnostic procedures.

Specifications

General

UNSPSC Code	12352204
Enzyme Commission (EC) Number	3.5.4.4 ( BRENDA \| IUBMB )
NACRES	NA.54
biological source	bovine (calf) intestine
form	solution
specific activity	~200 units/mg protein (At 25 °C with adenosine as the substrate.)
mol wt	230-440 kDa (high)
packaging	pkg of 2 mL (10 mg)
manufacturer/tradename	Roche
optimum pH	7.0-7.4
shipped in	wet ice
storage temp.	2-8°C

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Merck, 10102105001, Adenosine Deaminase (ADA)

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